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KMID : 0359320090490040285
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Korean Journal of Veterinary Research
2009 Volume.49 No. 4 p.285 ~ p.290
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Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis
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Lee Ji-Hye
Kim Keun-Jung
Choi Seon-A
Li Xiaoxia
Kim Eun-Young
Kim Min-Kyu
Oh Hyun-Ju
Lee Byeong-Chun
Kim Hye-Jin
Park Byung-Kwon
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Abstract
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This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with 1.3×10(8) spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.
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KEYWORD
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frozen-thawed semen, intrauterine insemination, microsatellite, superfecundation
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